- Catalog number
- A02241
- Size / count
- 0.1 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as RIP and DAP kinase, are mediators of apoptosis. DAP kinase (DAPK) is pro-apoptotic calcium-regulated serine/threonine kinase containing death domain. Ectopic expression of DAPK induces cell death and suppresses oncogenic transformation. DAPK mediates IFNγ induced apoptosis. A novel DAP kinase-related protein was recently identified and designated DAPK2 and DRP-1. Ectopicly expressed DAPK2 induced apoptosis in various types of cells. DAPK has high sequence homology to ZIP kinase and DRAK1/2, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. The messenger RNA of DAPK2 is expressed in multiple human tissues.
- Synonyms
- DRP1, DRP-1, Death-associated protein kinase 2, DAP-kinase-related protein 1, DAP kinase 2
- Gene Name
- DAPK2
- UniProt
- Q9UIK4
- Host
- Rabbit
- Reactivity
- Human, Mouse, Rat
- Cross Reactivity
- DAPK2 has no cross responses to DAPK1.
- Immunogen
- DAPK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human DAPK2. This antigenic peptide is identical to the corresponding amino acids of mouse origin (1,2) . The immunogen is located within the last 50 amino acids of DAPK2.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in neutrophils and eosinophils (PubMed:24163421) . Isoform 2 is expressed in embryonic stem cells (at protein level) . Isoform 1 is ubiquitously expressed in all tissue types examined with high levels in heart, lung and skeletal muscle.
- Applications
- ELISA, WB, IHC-P
- Field of Research
- Apoptosis, Autophagy
- Purification
- DAPK2 Antibody is affinity chromatography purified via peptide column.
- Concentration
- 1 mg/mL
- Form
- Liquid
- Function
- Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302) . Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421) .
- Storage Conditions
- DAPK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Observed Molecular Weight
- 42898 MW
- Gene Name Synonym
- Death-associated protein kinase 2
- Subcellular Location
- Cytoplasm. Cytoplasmic vesicle, autophagosome lumen.
- Protein Name
- Death-associated protein kinase 2
- Isotype
- IgG
- Contents
- DAPK2 Antibody is supplied in PBS containing 0.02% sodium azide.
