Anti-EIF3A Antibody

About this product

Boster Bio Anti-EIF3A Antibody catalog # A02339. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A02339

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Eukaryotic translation initiation factor 3 subunit A

Gene Name

EIF3A

UniProt

Q14152

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein of human EIF3A (NP_003741.1) .

Clonality

Polyclonal

Applications

WB, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773) . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF- 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC) . The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632, PubMed:11169732) . The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773, PubMed:27462815) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

166569 MW

Gene Name Synonym

Eukaryotic translation initiation factor 3 subunit A

Subcellular Location

Cytoplasm

Protein Name

Eukaryotic translation initiation factor 3 subunit A

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2