Anti-PABPN1 Antibody

About this product

Boster Bio Anti-PABPN1 Antibody catalog # A02445. Tested in WB applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A02445

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Polyadenylate-binding protein 2; PABP-2; Poly (A) -binding protein 2; Nuclear poly (A) -binding protein 1; Poly (A) -binding protein II; PABII; Polyadenylate-binding nuclear protein 1; PABPN1; PAB2, PABP2

Gene Name

PABPN1

UniProt

Q86U42

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

A synthetic peptide of human PABPN1 (NP_004634.1) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitous.

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly (A) tail of 200-250 nt to the upstream cleavage product (By similarity) . Stimulates poly (A) polymerase (PAPOLA) conferring processivity on the poly (A) tail elongation reaction and controls also the poly (A) tail length (By similarity) . Increases the affinity of poly (A) polymerase for RNA (By similarity) . Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense- mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes (PubMed:11371506) . Binds to poly (A) and to poly (G) with high affinity (By similarity) . May protect the poly (A) tail from degradation (By similarity) . Subunit of the trimeric poly (A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly (A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters (PubMed:27871484) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

32749 MW

Applications Notes

WB: 1:500-1:1000

Gene Name Synonym

Polyadenylate-binding protein 2

Subcellular Location

Nucleus

Protein Name

Polyadenylate-binding protein 2

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2