Anti-Bit1 PTRH2 Antibody

About this product

Boster Bio Anti-Bit1 PTRH2 Antibody (Catalog # A02459) . Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A02459

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apoptosis induced by integrin detachment. Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process. Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination. It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE. Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin.

Synonyms

BIT1, PTH2, CGI-147, BIT1, Peptidyl-tRNA hydrolase 2, mitochondrial, Bcl-2 inhibitor of transcription 1, PTH 2

Gene Name

PTRH2

UniProt

Q9Y3E5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Bit1 antibody was raised against a 15 amino acid synthetic peptide from near the amino-terminus of human Bit1. The immunogen is located within amino acids 20 - 70 of Bit1.

Clonality

Polyclonal

Tissue Specificity

Expressed at high levels in the small intestine, ovary, testis, kidney and endothelial cells.

Applications

ELISA, WB, ICC, IF

Field of Research

Apoptosis

Purification

Bit1 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis. .

Storage Conditions

Bit1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

19194 MW

Gene Name Synonym

Peptidyl-tRNA hydrolase 2

Subcellular Location

Mitochondrion.

Protein Name

Peptidyl-tRNA hydrolase 2

Isotype

IgG

Contents

Bit1 Antibody is supplied in PBS containing 0.02% sodium azide.