- Catalog number
- A02478
- Size / count
- 0.1 mg
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Nudel was initially discovered as a protein homologous to Aspergillus NUDE and that associated with Lis1 and polyprotein complex cytoplasmic dynein, both of which have been linked to neuronal development and migration. It was later shown to be a substrate of cdk5, a kinase known to be critical during neuronal migration; phosphorylation of Nudel by cdk5 affects its localization in neurons and affects neuritic morphology. It is thought that together with Lis1, Nudel regulates cytoplasmic dynein, a large polyprotein complex, in neuronal migration and mitosis through direct interactions. Similar interactions are thought to also play a role in membrane traffic in other cells as disruption of Nudel expression through RNA interference perturbed normal endomembrane flux and resulted in the fragmentation of the Golgi apparatus.
- Synonyms
- EOPA, NDE2, NUDEL, MITAP1, NDE1L1, EOPA, Nuclear distribution protein nudE-like 1, Mitosin-associated protein 1, Protein Nudel
- Gene Name
- NDEL1
- UniProt
- Q9GZM8
- Host
- Rabbit
- Reactivity
- Human, Mouse
- Immunogen
- Nudel antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Nudel. The immunogen is located within the first 50 amino acids of Nudel.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle.
- Applications
- ELISA, WB, ICC, IF
- Field of Research
- Neuroscience, Signal Transduction
- Purification
- Nudel Antibody is affinity chromatography purified via peptide column.
- Concentration
- 1 mg/mL
- Form
- Liquid
- Function
- Required for organization of the cellular microtubule array and microtubule anchoring at the centrosome. May regulate microtubule organization at least in part by targeting the microtubule severing protein KATNA1 to the centrosome. Also positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus ends. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the centripetal motion of secretory vesicles and the coupling of the nucleus and centrosome. Also required during brain development for the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Plays a role, together with DISC1, in the regulation of neurite outgrowth. Required for mitosis in some cell types but appears to be dispensible for mitosis in cortical neuronal progenitors, which instead requires NDE1. Facilitates the polymerization of neurofilaments from the individual subunits NEFH and NEFL. Positively regulates lysosome peripheral distribution and ruffled border formation in osteoclasts (By similarity) .
- Storage Conditions
- Nudel antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- Observed Molecular Weight
- 38375 MW
- Gene Name Synonym
- NudE nuclear distribution gene E homolog (A. nidulans) -like 1
- Subcellular Location
- Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Localizes to the cell body of the motor neurons and colocalizes with assembled neurofilaments within axonal processes. Localizes to the microtubules of the manchette in elongated spermatids. Colocalizes with DISC1 in the perinuclear region, including the centrosome (By similarity) . Localizes to the interphase centrosome and the mitotic spindle. Localizes to the kinetochore in a CENPF-dependent manner. .
- Prediction Reactivity
- Rat, Rabbit, Chicken
- Protein Name
- NudE nuclear distribution gene E homolog (A. nidulans) -like 1
- Isotype
- IgG
- Contents
- Nudel Antibody is supplied in PBS containing 0.02% sodium azide.
