Anti-EZH1 Antibody

About this product

Boster Bio Anti-EZH1 Antibody (Catalog # A02494) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A02494

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

EZH1 was initially identified as a homolog of the drosophila Enhancer of Zeste through transcription mapping of chromosome 17q21. Both EZH1 and the related protein EZH2 can form complexes with the noncanonical Polycomb repressive complex-2 (PRC2) and maintain repressive chromatin, but the PRC2-EZH1 complex mediates methylation of histone H3. Both EZH1 and EZH2 are thought to function in the maintenance of embryonic stem cell pluripotency and plasticity and recently have been shown to be essential for hair follicle homeostasis and wound repair.

Synonyms

KMT6B, KIAA0388, Histone-lysine N-methyltransferase EZH1, ENX-2

Gene Name

EZH1

UniProt

Q92800

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

Multiple isoforms of EZH1 are known to exist. EZH1 antibody is predicted to not cross-react with EZH2

Immunogen

EZH1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human EZH1. The immunogen is located within amino acids 180 - 230 of EZH1.

Clonality

Polyclonal

Applications

ELISA, WB, IHC-P, IF

Field of Research

Stem Cells

Purification

EZH1 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

Storage Conditions

EZH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

85271 MW

Gene Name Synonym

Enhancer of zeste homolog 1

Subcellular Location

Nucleus

Prediction Reactivity

Bovine

Protein Name

Enhancer of zeste homolog 1

Isotype

IgG

Contents

EZH1 Antibody is supplied in PBS containing 0.02% sodium azide.