Anti-RNase L Antibody

About this product

Boster Bio Anti-RNase L Antibody catalog # A02521. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A02521

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

2-5A-dependent ribonuclease;2-5A-dependent RNase;3.1.26.-; Ribonuclease 4; Ribonuclease L; RNase L; RNASEL; RNS4

Gene Name

RNASEL

UniProt

Q05823

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human RNASEL (NP_066956.1) .

Clonality

Polyclonal

Tissue Specificity

Highly expressed in spleen and thymus followed by prostate, testis, uterus, small intestine, colon and peripheral blood leukocytes.

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Endoribonuclease that functions in the interferon (IFN) antiviral response. In INF treated and virus infected cells, RNASEL probably mediates its antiviral effects through a combination of direct cleavage of single-stranded viral RNAs, inhibition of protein synthesis through the degradation of rRNA, induction of apoptosis, and induction of other antiviral genes. RNASEL mediated apoptosis is the result of a JNK-dependent stress- response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. Therefore, activation of RNASEL could lead to elimination of virus infected cells under some circumstances. In the crosstalk between autophagy and apoptosis proposed to induce autophagy as an early stress response to small double-stranded RNA and at later stages of prolonged stress to activate caspase-dependent proteolytic cleavage of BECN1 to terminate autophagy and promote apoptosis (PubMed:26263979) . Might play a central role in the regulation of mRNA turnover (PubMed:11585831) . Cleaves 3' of UpNp dimers, with preference for UU and UA sequences, to sets of discrete products ranging from between 4 and 22 nucleotides in length.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

83533 MW

Applications Notes

WB: 1:500-1:2000

Gene Name Synonym

2-5A-dependent ribonuclease

Subcellular Location

Cytoplasm

Protein Name

2-5A-dependent ribonuclease

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2