Anti-SGK3 Antibody

About this product

Boster Bio Anti-SGK3 Antibody catalog # A02534. Tested in WB applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A02534

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Serine/threonine-protein kinase Sgk3;2.7.11.1; Cytokine-independent survival kinase; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like; SGK3; CISK, SGKL

Gene Name

SGK3

UniProt

Q96BR1

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Recombinant protein of human SGK3.

Clonality

Polyclonal

Tissue Specificity

Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER- positive breast tumors than ER-negative breast tumors.

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na (+) channels: SCNN1A/ENAC and SCN5A, K (+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca (2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na (+) /dicarboxylate cotransporter: SLC13A2/NADC1, Na (+) -dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na (+) /H (+) exchanger: SLC9A3/NHE3, and the Na (+) /K (+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

57108 MW

Applications Notes

WB: 1:500-1:2000

Gene Name Synonym

Serine/threonine-protein kinase Sgk3

Subcellular Location

Cytoplasmic vesicle . Early endosome. Recycling endosome. Endosomal localization is a prerequisite for complete kinase activity. It is essential for its colocalization with the kinase responsible for phosphorylating Ser-486 thus allowing PDPK1 phosphorylation of Thr-320 resulting in complete activation of SGK3. Localized in vesicle-like structures and in the early endosome. Colocalizes with SLC9A3/NHE3 in the recycling endosomes.

Protein Name

Serine/threonine-protein kinase Sgk3

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2