Anti-ATF5 Antibody

About this product

Boster Bio Anti-ATF5 Antibody catalog # A02792. Tested in WB, ICC/IF, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A02792

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Cyclic AMP-dependent transcription factor ATF-5; cAMP-dependent transcription factor ATF-5; Activating transcription factor 5; Transcription factor ATFx; ATF5; ATFX

Gene Name

ATF5

UniProt

Q9Y2D1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant protein

Clonality

Polyclonal

Tissue Specificity

Widely expressed with higher expression levels in liver.

Applications

WB, ICC, IF, IHC, IP

Purification

ProA affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'- GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation (PubMed:10373550, PubMed:15358120, PubMed:21212266, PubMed:20654631) . Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4 (PubMed:15358120) . Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate (By similarity) . Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation (By similarity) . Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes (By similarity) . May be involved in osteogenic differentiation (PubMed:22442021) . Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1 (PubMed:21791614) . Plays an anti- apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent (By similarity) . Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver (PubMed:18332083) . In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase (PubMed:22528486, PubMed:18701499) . May act as a negative regulator of IL1B transduction pathway in liver (PubMed:24379400) . Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members (PubMed:25512613) . Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation (PubMed:24216764) . Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material (PubMed:26213385) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

30674 MW

Gene Name Synonym

Cyclic AMP-dependent transcription factor ATF-5

Subcellular Location

Cytoplasm

Protein Name

Cyclic AMP-dependent transcription factor ATF-5

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2