Anti-Protein KIBRA WWC1 Antibody

About this product

Boster Bio Anti-Protein KIBRA WWC1 Antibody (Catalog # A02837) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A02837

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The WW and C2 domain containing 1 (WWC1) protein, also known as KIBRA, possesses two WW domains and an internal C2-like domain (1) . WWC1 was originally identified as a memory performance-associated protein in humans (2) and has recently been shown to be a novel regulator of the Hippo pathway (3) . WWC1 is phosphorylated by the mitotic kinases Aurora-A and –B (4), and in turn activates the Aurora kinases and is required for precise chromosome alignment during mitosis (5) .

Synonyms

WW and C2 domain containing 1, HBEBP3, HBEBP36, KIBRA, MEMRYQTL

Gene Name

WWC1

UniProt

Q8IX03

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

WWC1 antibody is human, mouse and rat reactive. At least three isoforms of WWC1 are known to exist; this antibody will detect all three isoforms.

Immunogen

WWC1 antibody was raised against an 18 amino acid peptide near the amino terminus of human WWC1. The immunogen is located within amino acids 60 - 110 of WWC1.

Clonality

Polyclonal

Tissue Specificity

Expressed in mammary epithelial cells and breast cancer cell lines. Found in the luminal epithelium surrounding the ducts in the normal breast. In the brain, expressed in somatodendritic compartment of neurons in the cortex and hippocampus and in the cerebellum it is found in the Purkinje cells and some granule cells (at protein level) . Detected in brain, heart, colon and kidney. In the kidney, expressed in glomerular podocytes, in some tubules and in the collecting duct.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Homeostasis, Neuroscience

Purification

WWC1 antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ. Plays a role in cognition and memory performance.

Storage Conditions

WWC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

125301 MW

Gene Name Synonym

WW and C2 domain containing 1

Subcellular Location

Cytoplasm. Cytoplasm, perinuclear region. Nucleus. Cell projection, ruffle membrane. Colocalizes with PRKCZ in the perinuclear region.

Protein Name

WW and C2 domain containing 1

Isotype

IgG

Contents

WWC1 antibody is supplied in PBS containing 0.02% sodium azide.