Anti-Cysteine protease ATG4B ATG4B Antibody

About this product

Boster Bio Anti-Cysteine protease ATG4B ATG4B Antibody (Catalog # A02885) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A02885

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) . ATG4B, also known as AUTL1, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3) . ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4) . ATG4B has been found to be a novel protective protein in inflammatory colitis (5) .

Synonyms

Autophagy related 4B cysteine peptidase, APG4B, APG4-B, AUTL1

Gene Name

ATG4B

UniProt

Q9Y4P1

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

ATG4B antibody is human, mouse and rat reactive. At least two isoforms of ATG4B are known to exist; this antibody will detect only the larger isoform. ATG4B is predicted to not cross-react with other ATG4 proteins.

Immunogen

ATG4B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ATG4B. The immunogen is located within the last 50 amino acids of ATG4B.

Clonality

Polyclonal

Tissue Specificity

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Autophagy

Purification

ATG4B antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C- terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Storage Conditions

ATG4B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

44294 MW

Gene Name Synonym

Autophagy related 4B, cysteine peptidase

Subcellular Location

Cytoplasm .

Prediction Reactivity

Bovine, Chicken

Protein Name

Autophagy related 4B, cysteine peptidase

Isotype

IgG

Contents

ATG4B antibody is supplied in PBS containing 0.02% sodium azide.