Anti-EPRS Antibody

About this product

Boster Bio Anti-EPRS Antibody catalog # A02967. Tested in WB, IHC applications. This antibody reacts with Human.

Specifications

Catalog number

A02967

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Bifunctional glutamate/proline--tRNA ligase; Bifunctional aminoacyl-tRNA synthetase; Cell proliferation-inducing gene 32 protein; Glutamatyl-prolyl-tRNA synthetase; Glutamate--tRNA ligase;6.1.1.17; Glutamyl-tRNA synthetase; GluRS; Proline--tRNA ligase;6.1.1.15

Gene Name

EPRS

UniProt

P07814

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic peptide of human EPRS (NP_004437.2) .

Clonality

Polyclonal

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA (PubMed:1756734, PubMed:24100331, PubMed:23263184) . The phosphorylation of EPRS, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS function from protein synthesis to translation inhibition (PubMed:15479637, PubMed:23071094) . Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan (PubMed:28178239) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

170591 MW

Gene Name Synonym

Bifunctional glutamate/proline--tRNA ligase

Subcellular Location

Cytoplasm, cytosol

Protein Name

Bifunctional glutamate/proline--tRNA ligase

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2