Anti-Cullin-2 Cul2 Antibody

About this product

Boster Bio Anti-Cullin-2 Cul2 Antibody (Catalog # A02986) . Tested in IHC, IP, WB applications. This antibody reacts with Human.

Specifications

Catalog number

A02986

Size / count

100 µL

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

Anti-Cul2 antibody can be used in western blotting, ELISA and IP. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 2 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul2 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul2 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul2 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul2 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.

Synonyms

Cul2, MGC131970, CUL, Cullin

Gene Name

CUL2

UniProt

Q13617

Host

Rabbit

Reactivity

Human

Immunogen

Anti-Cul2 was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminus region near amino acids 720-745 of Human Cul2 coupled to KLH.

Clonality

Polyclonal

Applications

ELISA, IHC, IP, WB

Purification

Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 2. Cross-reactivity with Cul2 from other sources has not been determined.

Concentration

85 mg/mL by Refractometry

Form

Liquid (sterile filtered)

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store Cul2 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Isotype

Antiserum

Contents

0.01% (w/v) Sodium Azide