Anti-p33 ING1 Antibody

About this product

Boster Bio Anti-p33 ING1 Antibody (Catalog # A02999) . Tested in ELISA, WB applications. This antibody reacts with Human.

Specifications

Catalog number

A02999

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

P33 ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53-signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. The accession number listed below is for variant (4) that encodes the longest isoform (D) . Other synonyms for p33 ING1 include p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, growth inhibitor ING1, inhibitor of growth 1, tumor suppressor ING1 and growth inhibitory protein ING1.

Synonyms

P33 ING1, p33ING1, ING-1, ING 1, Growth inhibitory protein ING1, Homo sapiens growth inhibitor p33ING1 (ING1) mRNA, complete cds, ING 1, Inhibitor of growth 1, Inhibitor of growth family member 1

Gene Name

ING1

UniProt

Q9UK53

Host

Goat

Reactivity

Human

Immunogen

This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human p33 ING1 protein (Inhibitor of growth family, member 1) .

Clonality

Polyclonal

Tissue Specificity

Isoform 2 was expressed in all normal tissues and cells examined, as well as in all breast cancer and melanoma cell lines examined. Isoform 3 was expressed in testis, liver, and kidney, weakly expressed in colon and brain and not expressed in breast and cultured melanocytes. Isoform 4 was highly expressed in testis and weakly expressed in brain, but not expressed in breast, colon, kidney, melanocytes, breast cancer or melanoma cell lines.

Applications

ELISA, WB

Field of Research

Acetylation, Apoptosis, Cancer, Cancer Susceptibility, Cell Biology, Chromatin Modifying Enzymes, Epigenetics and Nuclear Signaling, Intracellular, Oncoproteins/Suppressors, p53 Pathway, Transcription, Tumor Suppressors

Purification

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p33 ING1 protein and is 100% homologous to all splice variants for ING1. Cross-reactivity with p33 ING1 protein from mouse and rat sources is expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p33 ING1 proteins from other sources is not known.

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Function

Cooperates with p53/TP53 in the negative regulatory pathway of cell growth by modulating p53-dependent transcriptional activation. Implicated as a tumor suppressor gene.

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

46738 MW

Gene Name Synonym

Inhibitor of growth protein 1

Subcellular Location

Nucleus.

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide