Anti-BUB3 (H274) Antibody

About this product

Boster Bio Anti-BUB3 (H274) Antibody catalog # A03118. Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A03118

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Mitotic checkpoint protein BUB3; BUB3

Gene Name

BUB3

UniProt

O43684

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 251-300 of Human BUB3.

Clonality

Polyclonal

Applications

WB, IHC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

37155 MW

Gene Name Synonym

Mitotic checkpoint protein BUB3

Subcellular Location

Nucleus. Chromosome, centromere, kinetochore . Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition. .

Protein Name

Mitotic checkpoint protein BUB3

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2