Anti-Centromere protein F CENPF Antibody

About this product

Boster Bio Anti-Centromere protein F CENPF Antibody catalog # A03311. Tested in IHC, IF applications. This antibody reacts with Human.

Specifications

Catalog number

A03311

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Centromere protein F; CENP-F; AH antigen; Kinetochore protein CENPF; Mitosin; CENPF

Gene Name

CENPF

UniProt

P49454

Host

Rabbit

Reactivity

Human

Immunogen

Synthesized peptide derived from human protein at AA range: 2190-2270

Clonality

Polyclonal

Applications

IHC, IF

Purification

CENPF antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/mL

Form

Liquid

Function

Required for kinetochore function and chromosome segregation in mitosis. Required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1. Regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation. May play a regulatory or permissive role in the normal embryonic cardiomyocyte cell cycle and in promoting continued mitosis in transformed, abnormally dividing neonatal cardiomyocytes. Interaction with RB directs embryonic stem cells toward a cardiac lineage. Involved in the regulation of DNA synthesis and hence cell cycle progression, via its C-terminus. Has a potential role regulating skeletal myogenesis and in cell differentiation in embryogenesis. Involved in dendritic cell regulation of T-cell immunity against chlamydia.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

367764 MW

Gene Name Synonym

Centromere protein F

Subcellular Location

Cytoplasm, perinuclear region. Nucleus matrix. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Relocalizes to the kinetochore/centromere (coronal surface of the outer plate) and the spindle during mitosis. Observed in nucleus during interphase but not in the nucleolus. At metaphase becomes localized to areas including kinetochore and mitotic apparatus as well as cytoplasm. By telophase, is concentrated within the intracellular bridge at either side of the mid-body.

Protein Name

Centromere protein F

Isotype

IgG

Contents

Liquid form in PBS containing 50% glycerol, and 0.02% sodium azide.