Anti-Siah-2 (E266) Antibody

About this product

Boster Bio Anti-Siah-2 (E266) Antibody catalog # A03379. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A03379

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

E3 ubiquitin-protein ligase SIAH2;2.3.2.27; RING-type E3 ubiquitin transferase SIAH2 ; Seven in absentia homolog 2; Siah-2; hSiah2; SIAH2

Gene Name

SIAH2

UniProt

O43255

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding to amino acids 241-290 of Human Siah-2.

Clonality

Polyclonal

Tissue Specificity

Widely expressed at low level.

Applications

IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (GPS2, POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP) . Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia. It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin- mediated degradation of TRAF2, whereas SIAH1 does not. Promotes monoubiquitination of SNCA. Regulates cellular clock function via ubiquitination of the circadian transcriptional repressors NR1D1 and NR1D2 leading to their proteasomal degradation. Plays an important role in mediating the rhythmic degradation/clearance of NR1D1 and NR1D2 contributing to their circadian profile of protein abundance (PubMed:26392558) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

34615 MW

Applications Notes

IHC: 1:50-1:200

Gene Name Synonym

E3 ubiquitin-protein ligase SIAH2

Subcellular Location

Cytoplasm

Protein Name

E3 ubiquitin-protein ligase SIAH2

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2