Anti-RNF20 Antibody

About this product

Boster Bio Anti-RNF20 Antibody (Catalog # A03457) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A03457

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

RNF20 is an E3 ubiquitin ligase that regulates chromosome structure by monoubiquitinating histone H2B. RNF20 selectively suppresses the expression of some genes such as several protooncogenes and growth-related genes, including many genes that are induced by epidermal growth factor by interfering with chromatin recruitment of transcription elongation factor SII (TFIIS) . In contrast, RNF20 also positively regulates the p53 tumor suppressor as well as numerous histone H2A and H2B genes. Together with the deubiquitinase USP44, RNF20 regulates stem cell differentiation by modulating H2B monoubiquitination.

Synonyms

BRE1, BRE1A, hBRE1, E3 ubiquitin-protein ligase BRE1A, RING finger protein 20, BRE1-A

Gene Name

RNF20

UniProt

Q5VTR2

Host

Rabbit

Reactivity

Human, Mouse

Cross Reactivity

Multiple isoforms of RNF20 are known to exist.

Immunogen

RNF20 antibody was raised against a 19 amino acid peptide near the amino terminus of human RNF20. The immunogen is located within amino acids 130 - 180 of RNF20.

Clonality

Polyclonal

Tissue Specificity

Expressed in the normal brain and also in malignant gliomas (at protein level) .

Applications

ELISA, WB, IHC-P, IF

Field of Research

Stem Cells

Purification

RNF20 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1) . H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively) . It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.

Storage Conditions

RNF20 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

113662 MW

Gene Name Synonym

Ring finger protein 20, E3 ubiquitin protein ligase

Subcellular Location

Nucleus

Prediction Reactivity

Bovine, Chicken

Protein Name

Ring finger protein 20, E3 ubiquitin protein ligase

Isotype

IgG

Contents

RNF20 Antibody is supplied in PBS containing 0.02% sodium azide.