Anti-ATG14 Antibody

About this product

Boster Bio Anti-ATG14 Antibody (Catalog # A03546) . Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A03546

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.

Synonyms

ATG14L, BARKOR, KIAA0831, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, Barkor

Gene Name

ATG14

UniProt

Q6ZNE5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

At least three isoforms of ATG14 are known to exist; this antibody will detect all three isoforms

Immunogen

ATG14 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG14. The immunogen is located within amino acids 270 - 320 of ATG14.

Clonality

Polyclonal

Applications

ELISA, WB, IF

Field of Research

Autophagy

Purification

ATG14 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071) . Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597) . Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597) . Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393) . Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393) . Binds to STX17-SNAP29 binary t- SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604) . Modulates the hepatic lipid metabolism (By similarity) .

Storage Conditions

ATG14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

55309 MW

Gene Name Synonym

ATG14 autophagy related 14 homolog (S. cerevisiae)

Subcellular Location

Cytoplasm

Protein Name

ATG14 autophagy related 14 homolog (S. cerevisiae)

Isotype

IgG

Contents

ATG14 Antibody is supplied in PBS containing 0.02% sodium azide.