Anti-GRF-1 (phospho-Y1087) ARHGAP35 Antibody

About this product

Boster Bio Anti-GRF-1 (phospho-Y1087) ARHGAP35 Antibody catalog # A03592Y1087. Tested in WB, IHC applications. This antibody reacts with Human.

Specifications

Catalog number

A03592Y1087

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Rho GTPase-activating protein 35

Gene Name

ARHGAP35

UniProt

Q9NRY4

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic phosphopeptide derived from human GRF-1 around the phosphorylation site of Tyrosine 1087.

Clonality

Polyclonal

Tissue Specificity

Detected in neutrophils (at protein level) .

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Rho GTPase-activating protein (GAP) (PubMed:19673492, PubMed:28894085) . Binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed:19673492) . This binding is inhibited by phosphorylation by PRKCA (PubMed:19673492) . Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity) . Transduces signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP) (By similarity) . Transduces SRC-dependent signals from cell-surface adhesion molecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity) . Modulates Rho GTPase-dependent F-actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity) . During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity) . Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed:1894621) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

170514 MW

Gene Name Synonym

Rho GTPase-activating protein 35

Subcellular Location

Cytoplasm, cytoskeleton, cilium basal body

Protein Name

Rho GTPase-activating protein 35

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2