Anti-GGA3 Antibody

About this product

Boster Bio Anti-GGA3 Antibody (Catalog # A03641) . Tested in ELISA, WB applications. This antibody reacts with Human, Chimpanzee.

Specifications

Catalog number

A03641

Size / count

100 µg

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

GGA3 (also known as ADP-ribosylation factor binding protein GGA3, Golgi-localized, gamma ear-containing, ARF-binding protein 3, Gamma-adaptin related protein 3) plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. This protein mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. GGA1 is a monomeric protein that interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200, GGA1 and GGA2 as well as RABEP1, RABGEF1 and type-I membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1. GGA3 is a ubiquitously expressed membrane-associated protein that is localized to TGN and endosomes. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Synonyms

GGA3, GGA-3, GGA 3, ADP-ribosylation factor-binding protein GGA3, Golgi-localized gamma ear-containing ARF-binding protein 3

Gene Name

GGA3

UniProt

Q9NZ52

Host

Rabbit

Reactivity

Human, Chimpanzee

Immunogen

This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 400-425 of Human GGA3.

Clonality

Polyclonal

Applications

ELISA, IP, WB

Field of Research

Golgi Proteins, Protein Trafficking, Signal Transduction

Purification

This affinity purified antibody is directed against human GGA3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross-reactivity may occur with GGA3 from macaque (93% homology) and orangutan (87% homology) sources. The immunizing sequence is present on both the long (isoform 1) and short (isoform 2) transcript variants for this protein.

Concentration

1.0mg/mL by UV absorbance at 280 nm

Form

Liquid (sterile filtered)

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide