Anti-Smac/Diablo Antibody

About this product

Boster Bio Anti-Smac/Diablo Antibody (Catalog # A03790) . Tested in WB applications. This antibody reacts with Human.

Specifications

Catalog number

A03790

Size / count

100 µL

Dry ice

Yes

Hazardous

No

Discontinued

No

Background

Apoptosis is a conserved cell suicide program essential for the development and homeostasis of multi-cellular organisms. Abnormal inhibition of apoptosis is a hallmark of cancer and autoimmune diseases, whereas excessive cell death is found in neurodegenerative disorders such as Alzheimers disease. Executioners of the apoptotic program are cysteine proteases termed caspases that exist as inactive zymogens in living cells and are activated during apoptosis. Active caspases cleave key intracellular protein substrates, resulting in the characteristic morphological changes associated with apoptosis. The release of cytochrome c from the mitochondria triggers the oligomerization of Apaf-1 in an ATP/dATP-dependent manner and induces the autoactivation of caspase-9. Active caspase-9 in turn activates downstream effector caspases including caspase -3, -6 and -7.

Synonyms

Smac, Diablo, Diablo homolog mitochondrial, Second mitochondria-derived activator of caspase, Smac protein

Gene Name

DIABLO

UniProt

Q9NR28

Host

Rabbit

Reactivity

Human

Cross Reactivity

No cross reactivity with other proteins.

Immunogen

This whole rabbit serum was prepared by repeated immunizations with recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239) .

Clonality

Polyclonal

Tissue Specificity

Highest expression found in heart, liver, kidney and testis.

Applications

ELISA, IP, WB

Field of Research

Apoptosis, Cancer, Cell Biology, Cell Death, Invasion/Microenvironment, Metabolism, Metabolism Processes, Mitochondrial, Mitochondrial Markers, Mitochondrial Metabolism, Pathways and Processes, Receptors

Purification

This antiserum is directed against human Smac/DIABLO and is useful in determining its presence in various assays.

Concentration

85 mg/mL by Refractometry

Form

Liquid (sterile filtered)

Function

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP) . Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases (By similarity) .

Shipping Conditions

Dry Ice Shipping

Storage Conditions

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Observed Molecular Weight

26820 MW

Gene Name Synonym

Diablo homolog, mitochondrial

Subcellular Location

Mitochondrion. Released into the cytosol when cells undergo apoptosis.

Isotype

Antiserum

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide