Anti-FAS-associated factor 1 FAF1 Antibody

About this product

Boster Bio Anti-FAS-associated factor 1 FAF1 Antibody (Catalog # A03842) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A03842

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fas-associated protein 1 (FAF1) was initially identified as a Fas-binding pro-apoptotic protein that is component of the death-inducing signaling complex in Fas-mediated apoptosis. FAF1 can also induce apoptosis in the absence of extrinsic death signals when overexpressed although it does not contain typical death motifs such as the death domain, death effector domain, and caspase recruitment domain. Overexpression of FAF1 also decreases the basal level of NF-κB activity in transfected 293 cells, inhibits NF-κB activity induced by TNF-α , IL-1β and lipopolysaccharide, and prevents NF-κB translocation to the nucleus, suggesting that another role of FAF1 is to negatively regulate the activity of NF-κB. FAF1 can also interact with the inflammatory signaling PYRIN-containing Apaf-1-like proteins (PYPAFs, also called NALPs) such as PYPAF1, PYPAF2 (NALP2), and PYPAF7, suggesting FAF1 may also be involved in the inflammation pathway. Multiple differentially spliced isoforms of FAF1 are known to exist.

Synonyms

HFAF1, CGI-03, HFAF1s, UBXD12, UBXN3A, FAS-associated factor 1, UBX domain-containing protein 12, hFAF1

Gene Name

FAF1

UniProt

Q9UNN5

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

FAF1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human FAF1. The immunogen is located within the last 50 amino acids of FAF1.

Clonality

Polyclonal

Tissue Specificity

Most abundant in testis, slightly less abundant in skeletal muscle and heart, followed by prostate, thymus, ovary, small intestine, and colon. Not detected in the peripheral blood leukocytes.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification

FAF1 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Ubiquitin-binding protein (PubMed:19722279) . Required for the progression of DNA replication forks by targeting DNA replication licensing factor CDT1 for degradation (PubMed:26842564) . Potentiates but cannot initiate FAS-induced apoptosis (By similarity) .

Storage Conditions

FAF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

73954 MW

Gene Name Synonym

Fas (TNFRSF6) associated factor 1

Subcellular Location

Nucleus

Protein Name

Fas (TNFRSF6) associated factor 1

Isotype

IgG

Contents

FAF1 Antibody is supplied in PBS containing 0.02% sodium azide.