Anti-UBE2D3 Antibody

About this product

Boster Bio Anti-UBE2D3 Antibody catalog # A03923. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A03923

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Ubiquitin-conjugating enzyme E2 D3;2.3.2.23

Gene Name

UBE2D3

UniProt

P61077

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide of human UBE2D3 (NP_003331.1) .

Clonality

Polyclonal

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys- 11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF (FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA- linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML- NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

16687 MW

Applications Notes

WB: 1:500-1:2000

Gene Name Synonym

Ubiquitin-conjugating enzyme E2 D3

Subcellular Location

Cell membrane

Protein Name

Ubiquitin-conjugating enzyme E2 D3

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2