- Catalog number
- A04214
- Size / count
- 100 µg
- Dry ice
- Yes
- Hazardous
- No
- Discontinued
- No
- Background
- DNMT 3L is a nuclear protein that has similarity to DNA methyltransferases, involved in de novo methylation of CpG islands. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
- Synonyms
- DNMT3L, DNA (cytosine-5) -methyltransferase 3-like, DNMT3L, DNMT 3L, DNMT-3L
- Gene Name
- DNMT3L
- UniProt
- Q9UJW3
- Host
- Rabbit
- Reactivity
- Human
- Immunogen
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 150-175 of Human DNMT3L (DNA (cytosine-5-) -methyltransferase 3-like) . DNMT3L is a nuclear protein with similarity to DNA methyltransferases.
- Clonality
- Polyclonal
- Tissue Specificity
- Expressed at low levels in several tissues including testis, ovary, and thymus.
- Applications
- ELISA, IHC, IP, WB
- Field of Research
- Epigenetics and Nuclear Signaling
- Purification
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. This antibody is expected to cross-react with mouse as the sequence of the human immunogen shows 76% identity with mouse. Reactivity with DNMT3L from other species has not yet been tested.
- Concentration
- 1.11 mg/mL by UV absorbance at 280 nm
- Form
- Liquid (sterile filtered)
- Function
- Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
- Shipping Conditions
- Dry Ice Shipping
- Storage Conditions
- Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
- Observed Molecular Weight
- 43583 MW
- Gene Name Synonym
- DNA (cytosine-5) -methyltransferase 3-like
- Subcellular Location
- Nucleus.
- Isotype
- IgG
- Contents
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
