- Catalog number
- A04320
- Size / count
- 2 mL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTSalpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.
- Synonyms
- Thymidylate Synthase, HsT422, MGC88736, TMS, TS, TSase, TYMS, TYMS protein
- Gene Name
- TYMS
- UniProt
- P04818
- Host
- Sheep
- Reactivity
- Human
- Immunogen
- This whole sheep serum was prepared by repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
- Clonality
- Polyclonal
- Applications
- ELISA, IHC, WB
- Field of Research
- DNA/RNA, DNA Synthesis, Epigenetics and Nuclear Signaling, Metabolic Signaling Pathways, Metabolism, Nucleotide Metabolism, Pathways and Processes
- Purification
- This antiserum is directed against human Thymidylate Synthase and is useful in determining its presence in various assays. Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
- Concentration
- 85 mg/mL by Refractometry
- Form
- Lyophilized
- Reconstitution
- Restore with deionized water (or equivalent) for reconstitution volume of 2.0 mL
- Function
- Contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
- Storage Conditions
- Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)
- Observed Molecular Weight
- 35716 MW
- Gene Name Synonym
- Thymidylate synthase
- Subcellular Location
- Nucleus. Cytoplasm. Mitochondrion. Mitochondrion matrix. Mitochondrion inner membrane.
- Isotype
- Antiserum
- Contents
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
