Anti-CD3-Delta CD3D Antibody

About this product

Boster Bio Anti-CD3-Delta CD3D Antibody catalog # A04405. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Rat.

Specifications

Catalog number

A04405

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

T-cell surface glycoprotein CD3 delta chain; T-cell receptor T3 delta chain; CD3d; CD3D; T3D

Gene Name

CD3D

UniProt

P04234

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant fusion protein of human CD3D (NP_000723.1) .

Clonality

Polyclonal

Tissue Specificity

CD3D is mostly present on T-lymphocytes with its TCR-CD3 partners. Present also in fetal NK-cells.

Applications

WB, IHC, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed:2470098) . In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells (PubMed:12215456) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

18930 MW

Gene Name Synonym

T-cell surface glycoprotein CD3 delta chain

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Name

T-cell surface glycoprotein CD3 delta chain

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2