Anti-AGAP2 Antibody

About this product

Boster Bio Anti-AGAP2 Antibody (Catalog # A04771) . Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A04771

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

AGAP2 (also known as Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. GTPase-activating protein (GAP) for ARF1 and ARF5, which also shows strong GTPase activity. It mediates anti-apoptotic effects of nerve growth factor by activating nuclear phosphoinositide 3-kinase. Isoform 1 participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It aids the coupling of metabotropic glutamate receptor 1 (GRM1) to cytoplasmic PI3 kinase by interacting with Homer scaffolding proteins, and seems to mediate anti-apoptotic effects of NGF by activating nuclear PI3 kinase. Isoform 2 does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. It also regulates the adapter protein 1 (AP-1) -dependent trafficking of proteins in the endosomal system. It seems to be oncogenic. It is overexpressed in cancer cells, prevents apoptosis and promotes cancer cell invasion. Anti-AGAP2 Antibody is useful for researchers interested in Developmental Biology, apoptosis, Cancer, Immunology, and Nuclear Signaling research.

Synonyms

AGAP2, AGAP-2, AGAP 2, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, ArfGAP with GTPase domain, ankyrin repeat and PH domain 2, centaurin gamma1, Centg1

Gene Name

AGAP2

UniProt

Q99490

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein.

Clonality

Polyclonal

Applications

ELISA, WB

Field of Research

Cell Cycle, Epigenetics and Nuclear Signaling, Kinases/Phosphatases, Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction

Purification

This protein A purified antibody is directed against human AGAP2 protein. The product was purified from monospecific antiserum by protein A chromatography. A BLAST analysis was used to suggest cross-reactivity with AGAP2 protein from mouse and rat based on 93% and 95% protein:protein homologies, respectively, with the immunizing sequence. Reactivity against homologues from other sources is not known.

Concentration

2.0 mg/mL by UV absorbance at 280 nm

Form

Lyophilized

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 μL

Storage Conditions

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Isotype

IgG

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide