Anti-COP1 RFWD2 Antibody

About this product

Boster Bio Anti-COP1 RFWD2 Antibody catalog # A04843. Tested in WB, ELISA applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A04843

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

E3 ubiquitin-protein ligase RFWD2;6.3.2.-; Constitutive photomorphogenesis protein 1 homolog; hCOP1; RING finger and WD repeat domain protein 2; RING finger protein 200; RFWD2; COP1, RNF200

Gene Name

RFWD2

UniProt

Q8NHY2

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human RFWD2. AA range:353-402

Tissue Specificity

Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. .

Applications

WB, ELISA

Purification

Immunogen affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

80474 MW

Gene Name Synonym

E3 ubiquitin-protein ligase RFWD2

Subcellular Location

Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.