Anti-Importin subunit alpha-3 KPNA4 Antibody

About this product

Boster Bio Anti-Importin subunit alpha-3 KPNA4 Antibody (Catalog # A05188) . Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A05188

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. Molecules containing the classical nuclear localization signal (NLS) are transported into the nucleus by alpha/beta heterodimers. KPNA3 has been shown to be important in the TNF-α -induced nuclear import of NF-κB. It is also involved in the stress-mediated nuclear stabilization of p53 and the nuclear import and replication of HIV-1 in both dividing and non-dividing cells.

Synonyms

QIP1, SRP3, IPOA3, QIP1, Importin subunit alpha-3, Importin alpha Q1, Qip1

Gene Name

KPNA4

UniProt

O00629

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

KPNA4 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human KPNA4. The immunogen is located within amino acids 30 - 80 of KPNA4.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in testis, ovary, small intestine, heart, skeletal muscle, lung and pancreas, but barely detectable in kidney, thymus, colon and peripheral blood leukocytes.

Applications

ELISA, WB, ICC, IF

Field of Research

Homeostasis

Purification

KPNA4 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non- classical NLS. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

Storage Conditions

KPNA4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

57887 MW

Gene Name Synonym

Karyopherin alpha 4 (importin alpha 3)

Subcellular Location

Cytoplasm . Nucleus .

Protein Name

Karyopherin alpha 4 (importin alpha 3)

Isotype

IgG

Contents

KPNA4 Antibody is supplied in PBS containing 0.02% sodium azide.