Anti-PNPase PNPT1 Antibody

About this product

Boster Bio Anti-PNPase PNPT1 Antibody catalog # A05460. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A05460

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Polyribonucleotide nucleotidyltransferase 1, mitochondrial;2.7.7.8;3'-5' RNA exonuclease OLD35; PNPase old-35; Polynucleotide phosphorylase 1; PNPase 1; Polynucleotide phosphorylase-like protein; PNPT1; PNPASE

Gene Name

PNPT1

UniProt

Q8TCS8

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human PNPT1. AA range:570-619

Clonality

Polyclonal

Applications

WB, IHC, IF, ELISA

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/mL

Form

Liquid

Function

RNA-binding protein implicated in numerous RNA metabolic processes. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'-to-5' direction. Mitochondrial intermembrane factor with RNA-processing exoribonulease activity. Component of the mitochondrial degradosome (mtEXO) complex, that degrades 3' overhang double- stranded RNA with a 3'-to-5' directionality in an ATP-dependent manner. Required for correct processing and polyadenylation of mitochondrial mRNAs. Plays a role as a cytoplasmic RNA import factor that mediates the translocation of small RNA components, like the 5S RNA, the RNA subunit of ribonuclease P and the mitochondrial RNA-processing (MRP) RNA, into the mitochondrial matrix. Plays a role in mitochondrial morphogenesis and respiration; regulates the expression of the electron transport chain (ETC) components at the mRNA and protein levels. In the cytoplasm, shows a 3'-to-5' exoribonuclease mediating mRNA degradation activity; degrades c-myc mRNA upon treatment with IFNB1/IFN-beta, resulting in a growth arrest in melanoma cells. Regulates the stability of specific mature miRNAs in melanoma cells; specifically and selectively degrades miR-221, preferentially. Plays also a role in RNA cell surveillance by cleaning up oxidized RNAs. Binds to the RNA subunit of ribonuclease P, MRP RNA and miR-221 microRNA.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

85951 MW

Gene Name Synonym

Polyribonucleotide nucleotidyltransferase 1, mitochondrial

Subcellular Location

Cytoplasm. Mitochondrion. Mitochondrion intermembrane space; Peripheral membrane protein.

Protein Name

Polyribonucleotide nucleotidyltransferase 1, mitochondrial

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.