Anti-HRI EIF2AK1 Antibody

About this product

Boster Bio Anti-HRI EIF2AK1 Antibody catalog # A05465. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.

Specifications

Catalog number

A05465

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Eukaryotic translation initiation factor 2-alpha kinase 1;2.7.11.1; Heme-controlled repressor; HCR; Heme-regulated eukaryotic initiation factor eIF-2-alpha kinase; Heme-regulated inhibitor; Hemin-sensitive initiation factor 2-alpha kinase; EIF2AK1; HRI, KIAA1369; PRO1362

Gene Name

EIF2AK1

UniProt

Q9BQI3

Host

Rabbit

Reactivity

Human, Mouse, Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human EIF2AK1. AA range:571-620

Tissue Specificity

Expressed predominantly in erythroid cells. At much lower levels, expressed in hepatocytes (at protein level) . .

Applications

WB, IHC, IF, ELISA

Purification

Immunogen affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

Inhibits protein synthesis at the translation initiation level, in response to various stress conditions, including oxidative stress, heme deficiency, osmotic shock and heat shock. Exerts its function through the phosphorylation of EIF2S1 at 'Ser- 48' and 'Ser-51', thus preventing its recycling. Binds hemin forming a 1:1 complex through a cysteine thiolate and histidine nitrogenous coordination. This binding occurs with moderate affinity, allowing it to sense the heme concentration within the cell. Thanks to this unique heme-sensing capacity, plays a crucial role to shut off protein synthesis during acute heme-deficient conditions. In red blood cells (RBCs), controls hemoglobin synthesis ensuring a coordinated regulation of the synthesis of its heme and globin moieties. Thus plays an essential protective role for RBC survival in anemias of iron deficiency. Similarly, in hepatocytes, involved in heme-mediated translational control of CYP2B and CYP3A and possibly other hepatic P450 cytochromes. May also contain ER stress during acute heme-deficient conditions (By similarity) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

71106 MW

Gene Name Synonym

Eukaryotic translation initiation factor 2-alpha kinase 1

Subcellular Location

Cytoplasm .

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.