Anti-RNF111 Antibody

About this product

Boster Bio Anti-RNF111 Antibody catalog # A05708. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A05708

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

E3 ubiquitin-protein ligase Arkadia

Gene Name

RNF111

UniProt

Q6ZNA4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding Human RNF111.

Clonality

Polyclonal

Tissue Specificity

Broadly expressed.

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

E3 ubiquitin-protein ligase (PubMed:26656854) . Required for mesoderm patterning during embryonic development (By similarity) . Acts as an enhancer of the transcriptional responses of the SMAD2/SMAD3 effectors, which are activated downstream of BMP (PubMed:14657019, PubMed:16601693) . Acts by mediating ubiquitination and degradation of SMAD inhibitors such as SMAD7, inducing their proteasomal degradation and thereby enhancing the transcriptional activity of TGF-beta and BMP (PubMed:14657019, PubMed:16601693) . In addition to enhance transcription of SMAD2/SMAD3 effectors, also regulates their turnover by mediating their ubiquitination and subsequent degradation, coupling their activation with degradation, thereby ensuring that only effectors 'in use' are degraded (By similarity) . Activates SMAD3/SMAD4- dependent transcription by triggering signal-induced degradation of SNON isoform of SKIL (PubMed:17591695) . Associates with UBE2D2 as an E2 enzyme (PubMed:22411132) . Specifically binds polysumoylated chains via SUMO interaction motifs (SIMs) and mediates ubiquitination of sumoylated substrates (PubMed:23751493) . Catalyzes 'Lys-63'-linked ubiquitination of sumoylated XPC in response to UV irradiation, promoting nucleotide excision repair (PubMed:23751493) . Mediates ubiquitination and degradation of sumoylated PML (By similarity) . The regulation of the BMP-SMAD signaling is however independent of sumoylation and is not dependent of SUMO interaction motifs (SIMs) (By similarity) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

108862 MW

Applications Notes

WB: 1:500-1:1000

Gene Name Synonym

E3 ubiquitin-protein ligase Arkadia

Subcellular Location

Nucleus

Protein Name

E3 ubiquitin-protein ligase Arkadia

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2