Anti-TRIAD3A RNF216 Antibody

About this product

Boster Bio Anti-TRIAD3A RNF216 Antibody (Catalog # A05841) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Specifications

Catalog number

A05841

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.

Synonyms

ZIN, CAHH, U7I1, TRIAD3, UBCE7IP1, ZIN, E3 ubiquitin-protein ligase RNF216, RING finger protein 216

Gene Name

RNF216

UniProt

Q9NWF9

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

TRIAD3A antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A. The immunogen is located within amino acids 120 - 170 of TRIAD3A.

Clonality

Polyclonal

Tissue Specificity

Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Innate Immunity

Purification

TRIAD3A Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down- regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3 inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis.

Storage Conditions

TRIAD3A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

99406 MW

Gene Name Synonym

Ring finger protein 216

Subcellular Location

Cytoplasm.

Protein Name

Ring finger protein 216

Isotype

IgG

Contents

TRIAD3A Antibody is supplied in PBS containing 0.02% sodium azide.