Anti-UBE2T Antibody

About this product

Boster Bio Anti-UBE2T Antibody catalog # A05874. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A05874

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Ubiquitin-conjugating enzyme E2 T;2.3.2.23

Gene Name

UBE2T

UniProt

Q9NPD8

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide, corresponding Human UBE2T.

Clonality

Polyclonal

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC) -induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784, PubMed:28437106) . Also mediates monoubiquitination of FANCL and FANCI (PubMed:16916645, PubMed:17938197, PubMed:19111657, PubMed:19589784) . May contribute to ubiquitination and degradation of BRCA1 (PubMed:19887602) . In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys- 63'-linked polyubiquitination (PubMed:20061386) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

22521 MW

Applications Notes

WB: 1:500-1:1000

Gene Name Synonym

Ubiquitin-conjugating enzyme E2 T

Subcellular Location

Nucleus

Protein Name

Ubiquitin-conjugating enzyme E2 T

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2