Anti-POLR3A Antibody

About this product

Boster Bio Anti-POLR3A Antibody catalog # A06059. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Monkey.

Specifications

Catalog number

A06059

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA-directed RNA polymerase III subunit RPC1; RNA polymerase III subunit C1;2.7.7.6; DNA-directed RNA polymerase III largest subunit; DNA-directed RNA polymerase III subunit A; RNA polymerase III 155 kDa subunit; RPC155; RNA polymerase III subunit C160; POLR3A

Gene Name

POLR3A

UniProt

O14802

Host

Rabbit

Reactivity

Human, Mouse, Monkey

Immunogen

The antiserum was produced against synthesized peptide derived from human RPC1. AA range:31-80

Tissue Specificity

Expressed in the brain, in the cortex and the white matter (at protein level) . .

Applications

WB, IHC, IF, ELISA

Purification

Immunogen affinity purified

Concentration

1 mg/mL

Form

Liquid

Function

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Forms the polymerase active center together with the second largest subunit. A single-stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity) . Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

155641 MW

Gene Name Synonym

DNA-directed RNA polymerase III subunit RPC1

Subcellular Location

Nucleus.

Isotype

IgG

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.