Anti-DNA helicase MCM8 MCM8 Antibody

About this product

Boster Bio Anti-DNA helicase MCM8 MCM8 Antibody catalog # A06148. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A06148

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

DNA helicase MCM8;3.6.4.12

Gene Name

MCM8

UniProt

Q9UJA3

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to residues in Human MCM8.

Clonality

Polyclonal

Tissue Specificity

Highest levels in placenta, lung and pancreas. Low levels in skeletal muscle and kidney. Expressed in various tumors with highest levels in colon and lung cancers.

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Component of the MCM8-MCM9 complex, a complex involved in the repair of double-stranded DNA breaks (DBSs) and DNA interstrand cross-links (ICLs) by homologous recombination (HR) (PubMed:23401855) . Required for DNA resection by the MRE11-RAD50- NBN/NBS1 (MRN) complex by recruiting the MRN complex to the repair site and by promoting the complex nuclease activity (PubMed:26215093) . Probably by regulating the localization of the MNR complex, indirectly regulates the recruitment of downstream effector RAD51 to DNA damage sites including DBSs and ICLs (PubMed:23401855) . The MCM8-MCM9 complex is dispensable for DNA replication and S phase progression (PubMed:23401855) . However, may play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G (1) phase by recruiting CDC6 to the origin recognition complex (ORC) (PubMed:15684404) . Probably by regulating HR, plays a key role during gametogenesis (By similarity) . Stabilizes MCM9 protein (PubMed:23401855, PubMed:26215093) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

93697 MW

Applications Notes

WB: 1:500-1:1000

Gene Name Synonym

DNA helicase MCM8

Subcellular Location

Nucleus

Protein Name

DNA helicase MCM8

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2