Anti-YTHDC1 Antibody

About this product

Boster Bio Anti-YTHDC1 Antibody catalog # A06172. Tested in WB, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A06172

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

YTH domain-containing protein 1 ; Splicing factor YT521

Gene Name

YTHDC1

UniProt

Q96MU7

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human YTHDC1 (NP_001026902.1) .

Clonality

Polyclonal

Applications

WB, ICC, IF

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A) -containing RNAs (PubMed:26318451, PubMed:26876937, PubMed:25242552, PubMed:28984244) . M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:26318451, PubMed:25242552) . Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937) . Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937) . In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937) . May also regulate alternative splice site selection (PubMed:20167602) . Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244) . Also recognizes and binds m6A on other RNA molecules (PubMed:27602518) . Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (PubMed:27602518) . Involved in S- adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

84700 MW

Gene Name Synonym

YTH domain-containing protein 1

Subcellular Location

Nucleus

Protein Name

YTH domain-containing protein 1

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2