Anti-SUPT6H Antibody

About this product

Boster Bio Anti-SUPT6H Antibody catalog # A06484. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A06484

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Transcription elongation factor SPT6; hSPT6; Histone chaperone suppressor of Ty6; Tat-cotransactivator 2 protein; Tat-CT2 protein; SUPT6H; KIAA0162, SPT6H

Gene Name

SUPT6H

UniProt

Q7KZ85

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human SUPT6H (NP_003161.2) .

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

WB, ICC, IF, IP

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Transcription elongation factor which binds histone H3 and plays a key role in the regulation of transcription elongation and mRNA processing. Enhances the transcription elongation by RNA polymerase II (RNAPII) and is also required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. Besides chaperoning histones in transcription, acts to transport and splice mRNA by forming a complex with IWS1 and the C-terminal domain (CTD) of the RNAPII subunit RPB1 (POLR2A) . The SUPT6H:IWS1:CTD complex recruits mRNA export factors (ALYREF/THOC4, EXOSC10) as well as histone modifying enzymes (such as SETD2), to ensure proper mRNA splicing, efficient mRNA export and elongation-coupled H3K36 methylation, a signature chromatin mark of active transcription. SUPT6H via its association with SETD1A, regulates both class-switch recombination and somatic hypermutation through formation of H3K4me3 epigenetic marks on activation-induced cytidine deaminase (AICDA) target loci. Promotes the activation of the myogenic gene program by entailing erasure of the repressive H3K27me3 epigenetic mark through stabilization of the chromatin interaction of the H3K27 demethylase KDM6A.

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

199073 MW

Gene Name Synonym

Transcription elongation factor SPT6

Subcellular Location

Nucleus .

Protein Name

Transcription elongation factor SPT6

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2