Anti-PIK3R4 Antibody

About this product

Boster Bio Anti-PIK3R4 Antibody (Catalog # A06618) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A06618

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

PIK3R4 (phosphoinositide-3-kinase regulatory subunit 4, also known as VPS15) is a 150kD adaptor protein that enhances the lipid kinase activity of PIK3 complexes and may be involved in regulating membrane trafficking late in the endocytic pathway and autophagy (1,2) . PIK3R4 is ubiquitously expressed and contains 7 WD repeats, 3 HEAT repeats and 1 protein kinase domain (1) . PIK3R4 is crucial for VPS34 function and is necessary for autophagosome formation in multicellular animals and reveal an important role of VPS15 in cellular protection against ubiquitin-positive protein aggregates (3-5) .

Synonyms

Phosphoinositide-3-kinase regulatory subunit 4, p150, Phosphoinositide 3-kinase adaptor protein, PI3-kinase p150 subunit, PI3-kinase regulatory subunit 4, VPS15

Gene Name

PIK3R4

UniProt

Q99570

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

PIK3R4 antibody is human, mouse and rat reactive.

Immunogen

PIK3R4 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PIK3R4. The immunogen is located within amino acids 1290 - 1340 of PIK3R4.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Autophagy

Purification

PIK3R4 antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Regulatory subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123) .

Storage Conditions

PIK3R4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

153103 MW

Gene Name Synonym

Phosphoinositide 3-kinase regulatory subunit 4

Subcellular Location

Late endosome

Protein Name

Phosphoinositide 3-kinase regulatory subunit 4

Isotype

IgG

Contents

PIK3R4 antibody is supplied in PBS containing 0.02% sodium azide.