Anti-Piwi-like protein 4 PIWIL4 Antibody

About this product

Boster Bio Anti-Piwi-like protein 4 PIWIL4 Antibody catalog # A06797. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A06797

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Piwi-like protein 4; PIWIL4; HIWI2, PIWI

Gene Name

PIWIL4

UniProt

Q7Z3Z4

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human PIWIL4 (NP_689644.2) .

Clonality

Polyclonal

Tissue Specificity

Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.

Applications

WB, IHC

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity (By similarity) . Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons (By similarity) . Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements (By similarity) . Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association (By similarity) . The piRNA process acts upstream of known mediators of DNA methylation (By similarity) . Does not show endonuclease activity (By similarity) . Plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-incompetent' component that loads cleaved piRNAs from the 'slicer-competent' component PIWIL2 and target them on genomic transposon loci in the nucleus (By similarity) . May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci (PubMed:17544373) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

96589 MW

Gene Name Synonym

Piwi-like protein 4

Subcellular Location

Nucleus

Protein Name

Piwi-like protein 4

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2