Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody

About this product

Boster Bio Anti-Pre-mRNA-splicing factor SPF27 BCAS2 Antibody (Catalog # A07113) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A07113

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

BCAS2 was identified through differential display analysis as an mRNA species that was overexpressed in MCF7 and BT-20 breast cancer cell lines. The chromosomal region containing this gene, 1p13.3021, is amplified in these cells lines. BCAS2 is a transcriptional cofactor that enhances estrogen receptor-mediated gene expression, and directly interacts with the tumor suppressor p53 to reduce p53 transcriptional activity by reducing p53 protein level in the absence of DNA damage. Deprivation of BCAS2 through RNA inhibition induces apoptosis in p53-wild type cells, but causes G2-M arrest in p53-null or -mutant cells; this effect was reversed with the expression of ectopic BCAS2. BCAS2 may thus be potentially useful as a therapeutic target in the treatment of cancer.

Synonyms

DAM1, SPF27, Snt309, DAM1, Pre-mRNA-splicing factor SPF27, Breast carcinoma-amplified sequence 2

Gene Name

BCAS2

UniProt

O75934

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

BCAS2 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human BCAS2. The immunogen is located within the last 50 amino acids of BCAS2.

Clonality

Polyclonal

Tissue Specificity

Ubiquitously expressed.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Cancer, Cell Cycle

Purification

BCAS2 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. May have a scaffolding role in the spliceosome assembly as it contacts all other components of the core complex. The PRP19-CDC5L complex may also play a role in the response to DNA damage (DDR) .

Storage Conditions

BCAS2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

26131 MW

Gene Name Synonym

Breast carcinoma amplified sequence 2

Subcellular Location

Nucleus, nucleolus

Protein Name

Breast carcinoma amplified sequence 2

Isotype

IgG

Contents

BCAS2 Antibody is supplied in PBS containing 0.02% sodium azide.