- Catalog number
- A07197-80ul
- Size / count
- 80 µL
- Dry ice
- No
- Hazardous
- No
- Discontinued
- No
- Background
- The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8 (GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.
- Synonyms
- Bardet-Biedl syndrome 5 protein, BBS5
- Gene Name
- BBS5
- UniProt
- Q8N3I7
- Host
- Rabbit
- Reactivity
- Human
- Immunogen
- This BBS5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 108-141 amino acids from the Central region of human BBS5.
- Clonality
- Polyclonal
- Applications
- WB
- Field of Research
- Neuroscience
- Purification
- This antibody is purified through a protein A column, followed by peptide affinity purification.
- Function
- The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. This ciliogenic function is mediated in part by the Rab8 GDP/GTP exchange factor, which localizes to the basal body and contacts the BBSome. Rab8 (GTP) enters the primary cilium and promotes extension of the ciliary membrane. Firstly the BBSome associates with the ciliary membrane and binds to RAB3IP/Rabin8, the guanosyl exchange factor (GEF) for Rab8 and then the Rab8-GTP localizes to the cilium and promotes docking and fusion of carrier vesicles to the base of the ciliary membrane. The BBSome complex, together with the LTZL1, controls SMO ciliary trafficking and contributes to the sonic hedgehog (SHH) pathway regulation. Required for BBSome complex ciliary localization but not for the proper complex assembly.
- Storage Conditions
- Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
- Observed Molecular Weight
- 38755 Da
- Applications Notes
- WB: 1:1000
- Subcellular Location
- Cell projection, cilium membrane. Cytoplasm. Cytoplasm, cytoskeleton, cilium basal body. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite. Note=Localizes to basal bodies.
- Prediction Reactivity
- Monkey, Mouse
- Isotype
- Rabbit IgG
- Contents
- Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
