Anti-Nanos homolog 1 Nanos1 Antibody

About this product

Boster Bio Anti-Nanos homolog 1 Nanos1 Antibody (Catalog # A07276) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Specifications

Catalog number

A07276

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Nanos1 is one of three known mammalian homologs to the Drosophila gene nanos. Nanos1 is an RNA-binding protein containing a zinc-finger motif and is expressed in the developing nervous system and continues in the adult brain. Interestingly, unlike mice deficient in either nanos2 or nanos3, mice lacking the nanos1 gene develop normally with no sign of abnormalities. Recently it has been found that expression of nanos1 mRNA is down-regulated by E-cadherin in a human breast cancer cell line and the amino-terminal domain on Nanos1 interacts with the E-cadherin-binding protein p120ctn. Furthermore, overexpression of Nanos1 in human colorectal DLD1 cancer cells functionally abolished cell-cell adhesion, allowing the cancer cells to develop strong migratory and invasive properties. These results suggest that targeting Nanos1 might prove an effective strategy in the treatment of E-cadherin-negative tumors.

Synonyms

NOS1, SPGF12, NOS1, Nanos homolog 1, NOS-1

Gene Name

NANOS1

UniProt

Q8WY41

Host

Rabbit

Reactivity

Human

Immunogen

Nanos1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Nanos1. The immunogen is located within the first 50 amino acids of Nanos1.

Clonality

Polyclonal

Tissue Specificity

Testis and ovary (at protein level) . Predominantly expressed in testis. Specifically expressed during germline development. In adult tissues, it is mainly expressed in spermatogonia, the stem cells of the germline. Also expressed during meiosis in spermatocytes. Not present in late, post-meiotic stage germ cells. Expressed in fetal ovaries, while it is weakly or not expressed in mature postmeiotic oocytes, suggesting that it may be expressed in premeiotic female germ cells. Expressed at high levels only in the E-cadherin deficient cell lines. Highly expressed in lung carcinomas and mostly detected in invasive tumor cells and its expression correlates with tumor aggressiveness.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Signal Transduction

Purification

Nanos1 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

May act as a translational repressor which regulates translation of specific mRNAs by forming a complex with PUM2 that associates with the 3'-UTR of mRNA targets. Capable of interfering with the proadhesive and anti-invasive functions of E-cadherin. Up-regulates the production of MMP14 to promote tumor cell invasion.

Storage Conditions

Nanos1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

30230 MW

Gene Name Synonym

Nanos homolog 1 (Drosophila)

Subcellular Location

Cytoplasm, perinuclear region

Prediction Reactivity

Mouse

Protein Name

Nanos homolog 1 (Drosophila)

Isotype

IgG

Contents

Nanos1 Antibody is supplied in PBS containing 0.02% sodium azide.