Anti-Importin subunit alpha-7 KPNA6 Antibody

About this product

Boster Bio Anti-Importin subunit alpha-7 KPNA6 Antibody (Catalog # A07295) . Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A07295

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Karyopherin, a cytosolic and heterodimeric protein complex consisting of alpha and beta subunits, is responsible for targeting proteins with nuclear localization signals to the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. The alpha subunit and imported substrate enter the nucleus and accumulate in the nucleoplasm, while the beta subunit accumulates at the NPC. KPNA6 belongs to a subfamily within the KPNA family that also includes IPOA5&6. Down-regulation of KPNA6 by RNAi expression in HeLa cells strongly inhibited cell proliferation, possibly due to blocking the nuclear import of specific factors essential for cell growth and proliferation. KPNA6 also interacts with the Rev protein of HIV-1 and promote its nuclear import.

Synonyms

IPOA7, KPNA7, IPOA7, Importin subunit alpha-7, Karyopherin subunit alpha-6

Gene Name

KPNA6

UniProt

O60684

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

KPNA6 antibody was raised against a 13 amino acid synthetic peptide near the amino terminus of human KPNA6. The immunogen is located within amino acids 70 - 120 of KPNA6.

Clonality

Polyclonal

Tissue Specificity

Widely expressed.

Applications

ELISA, WB, IF

Field of Research

Homeostasis

Purification

KPNA6 Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Storage Conditions

KPNA6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

60030 MW

Gene Name Synonym

Karyopherin alpha 6 (importin alpha 7)

Prediction Reactivity

Bovine

Protein Name

Karyopherin alpha 6 (importin alpha 7)

Isotype

IgG

Contents

KPNA6 Antibody is supplied in PBS containing 0.02% sodium azide.