Anti-RNAse H2A Antibody

About this product

Boster Bio Anti-RNAse H2A Antibody (Catalog # A07386) . Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A07386

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.

Synonyms

AGS4, JUNB, RNHL, RNHIA, RNASEHI, Ribonuclease H2 subunit A, Aicardi-Goutieres syndrome 4 protein, RNase H2 subunit A

Gene Name

RNASEH2A

UniProt

O75792

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

RNAse H2A antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A. The immunogen is located within amino acids 190 - 240 of RNAse H2A.

Clonality

Polyclonal

Tissue Specificity

Detected in heart, breast, prostate, skeletal muscle, testis, uterus, bone marrow, colon, small intestine, stomach and thymus.

Applications

ELISA, WB, ICC, IF

Field of Research

Homeostasis

Purification

RNAse H2A Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging- strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

Storage Conditions

RNAse H2A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Observed Molecular Weight

33395 MW

Gene Name Synonym

Ribonuclease H2, subunit A

Subcellular Location

Nucleus .

Prediction Reactivity

Bovine

Protein Name

Ribonuclease H2, subunit A

Isotype

IgG

Contents

RNAse H2A Antibody is supplied in PBS containing 0.02% sodium azide.