Anti-ULK3 Antibody

About this product

Boster Bio Anti-ULK3 Antibody (Catalog # A07706) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.

Specifications

Catalog number

A07706

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

ULK3 belongs to the Ser/Thr protein kinase superfamily and plays a role in the ATP-dependent phosphorylation of target proteins (1) . Knockout of ULK genes results in a severe defect in the autophagy pathway (2) . ULK3, like the other Unc-51-like kinases such as ULK1, ULK2 and ULK4, is highly conserved among eukaryotes (3) . ULK3 has been shown to be a positive regulator of the Hedgehog signaling pathway by enhancing GLI1 and GLI2 transcriptional activity (4) . Furthermore, ULK3 can also interact with SUFU, a protein required for the negative regulation of GLI proteins; this interaction blocks the autophosphorylation of ULK3 and blocks its ability to regulate the GLI proteins (5) .

Synonyms

Unc-51-like kinase 3

Gene Name

ULK3

UniProt

Q6PHR2

Host

Rabbit

Reactivity

Human

Cross Reactivity

ULK3 antibody is human specific. Multiple isoforms of ULK3 are known to exist.

Immunogen

ULK3 antibody was raised against a 19 amino acid peptide near the center of human ULK3. The immunogen is located within amino acids 230 - 280 of ULK3.

Clonality

Polyclonal

Tissue Specificity

Widely expressed. Highest levels observed in fetal brain. In adult tissues, high levels in brain, liver and kidney, moderate levels in testis and adrenal gland and low levels in heart, lung, stomach, thymus, prostate and placenta. In the brain, highest expression in the hippocampus, high levels also detected in the cerebellum, olfactory bulb and optic nerve. In the central nervous system, lowest levels in the spinal cord.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Autophagy

Purification

ULK3 antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Serine/threonine protein kinase that acts as a regulator of Sonic hedgehog (SHH) signaling and autophagy. Acts as a negative regulator of SHH signaling in the absence of SHH ligand: interacts with SUFU, thereby inactivating the protein kinase activity and preventing phosphorylation of GLI proteins (GLI1, GLI2 and/or GLI3) . Positively regulates SHH signaling in the presence of SHH: dissociates from SUFU, autophosphorylates and mediates phosphorylation of GLI2, activating it and promoting its nuclear translocation. Phosphorylates in vitro GLI2, as well as GLI1 and GLI3, although less efficiently. Also acts as a regulator of autophagy: following cellular senescence, able to induce autophagy.

Storage Conditions

ULK3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

53444 MW

Gene Name Synonym

Unc-51 like kinase 3

Subcellular Location

Cytoplasm

Prediction Reactivity

Rat, Mouse, Chicken

Protein Name

Unc-51 like kinase 3

Isotype

IgG

Contents

ULK3 antibody is supplied in PBS containing 0.02% sodium azide.