Anti-MECR Antibody

About this product

Boster Bio Anti-MECR Antibody (Catalog # A08189) . Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A08189

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The mitochondrial trans-2-enoyl-CoA reductase (MECR), was initially identified as nuclear receptor-binding factor 1 (NRBF1), which can interact with a multitude of nuclear hormone receptors in the presence of the respective ligands. MECR has been shown to be part of the mitochondrial fatty acid synthesis (FAS II) system and to catalyze the NAPDH-dependent reduction of 2-enoyl thioesters, generating saturated acyl-groups. Overexpression of this gene in transgenic mice can lead to cardiac abnormalities, suggesting that inappropriate expression of genes of FAS II can result in the development of hereditary cardiomyopathy.

Synonyms

NRBF1, CGI-63, FASN2B, NBRF1, Trans-2-enoyl-CoA reductase, mitochondrial, Nuclear receptor-binding factor 1, HsNrbf-1

Gene Name

MECR

UniProt

Q9BV79

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

At least two isoforms of MECR are known to exist; this antibody will detect both isoforms.

Immunogen

Rabbit polyclonal MECR antibody was raised against a 15 amino acid peptide near the carboxy terminus of human MECR. The immunogen is located within amino acids 300 - 350 of MECR.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in skeletal and heart muscle. Expressed at lower level in placenta, liver, kidney and pancreas. Weakly or not expressed in lung.

Applications

ELISA, WB

Field of Research

Homeostasis

Purification

MECR Antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Catalyzes the NADPH-dependent reduction of trans-2-enoyl thioesters in mitochondrial fatty acid synthesis (fatty acid synthesis type II) . Fatty acid chain elongation in mitochondria uses acyl carrier protein (ACP) as an acyl group carrier, but the enzyme accepts both ACP and CoA thioesters as substrates in vitro. Has a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2-decenoyl-CoA (C10), and trans- 2-hexadecenoyl-CoA (C16) .

Storage Conditions

MECR antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

40462 MW

Gene Name Synonym

Mitochondrial trans-2-enoyl-CoA reductase

Subcellular Location

Isoform 1: Mitochondrion

Prediction Reactivity

Bovine

Protein Name

Mitochondrial trans-2-enoyl-CoA reductase

Isotype

IgG

Contents

MECR Antibody is supplied in PBS containing 0.02% sodium azide.