Anti-MGAT3 Antibody

About this product

Boster Bio Anti-MGAT3 Antibody catalog # A08470. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A08470

Size / count

100 µL

Dry ice

No

Hazardous

No

Discontinued

No

Synonyms

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase ;2.4.1.144

Gene Name

MGAT3

UniProt

Q09327

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein of human MGAT3 (NP_002400.3) .

Clonality

Polyclonal

Applications

WB

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Concentration

1 mg/mL

Form

Liquid

Function

It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains, called bisecting N-acetylglucosamine (GlcNAc) . It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. The addition of this bisecting GlcNAc residue alters not only the composition, but also the conformation of the N-glycan. The introduction of the bisecting GlcNAc residue results in the suppression of further processing and elongation of N-glycans, precluding the formation of beta-1,6 GlcNAc branching, catalyzed by MGAT5 since it is unable to use the bisected oligosaccharide as a substrate (PubMed:19403558) . Addition of bisecting N-acetylglucosamine to CDH1/E-cadherin modulates CDH1 cell membrane location (PubMed:19403558) . Inhibits NeuAc-alpha-2,3-Gal-beta-1,4- GlcNAc- formation which modulates sialylation levels and plays a role in cell migration regulation (PubMed:26801611) . In brain, addition of bisecting N-acetylglucosamine to BACE1 blocks its lysosomal targeting in response to oxidative stress and further degradation which increases its location to early endosome and the APP cleavage (By similarity) .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

61313 MW

Applications Notes

WB: 1:500-1:2000

Gene Name Synonym

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Subcellular Location

Golgi apparatus membrane; Single-pass type II membrane protein .

Protein Name

Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase

Contents

Rabbit IgG, 1mg/mL in PBS with 0.02% sodium azide, 50% glycerol, pH7.2