Anti-Cysteine protease ATG4C ATG4C Antibody

About this product

Boster Bio Anti-Cysteine protease ATG4C ATG4C Antibody (Catalog # A09728) . Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Specifications

Catalog number

A09728

Size / count

0.1 mg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) . ATG4C, also known as autophagin-3, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3) . Recent studies have shown that ATG4C and Beclin-1 are regulated by the microRNA miR-376b; overexpression of miR-376b led to decreased mRNA and protein levels, thereby blocking starvation and TOR inhibition-related autophagy (4) .

Synonyms

APG4C, AUTL1, AUTL3, APG4-C, APG4C, Cysteine protease ATG4C, AUT-like 3 cysteine endopeptidase

Gene Name

ATG4C

UniProt

Q96DT6

Host

Rabbit

Reactivity

Human, Mouse, Rat

Cross Reactivity

ATG4C antibody is human, mouse and rat reactive. ATG4C is predicted to not cross-react with other ATG4 proteins.

Immunogen

ATG4C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4C. The immunogen is located within the last 50 amino acids of ATG4C.

Clonality

Polyclonal

Tissue Specificity

Highly expressed in skeletal muscle, heart, liver and testis.

Applications

ELISA, WB, IHC-P, IF

Field of Research

Autophagy

Purification

ATG4C antibody is affinity chromatography purified via peptide column.

Concentration

1 mg/mL

Form

Liquid

Function

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity) . Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Storage Conditions

ATG4C antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Observed Molecular Weight

52497 MW

Gene Name Synonym

Autophagy related 4C, cysteine peptidase

Subcellular Location

Cytoplasm .

Protein Name

Autophagy related 4C, cysteine peptidase

Isotype

IgG

Contents

ATG4C antibody is supplied in PBS containing 0.02% sodium azide.